(C) Nterminally HAtagged wildtype (WT) FUS and the indicated point mutants were transiently expressed in HeLa cells; 24 h posttransfection cells were stained with an HAspecific antibody (green), a nuclear counterstain (blue) and analysed by confocal microscopy. Although the WT protein is located almost exclusively in the nucleus, deletion of the Cterminal 13 amino acids ( or replacement of arginine residues by alanine (R521A/R522A/R524A or R514A/R518A) leads to a predominantly cytosolic localization. Scale bar, 20 (D) Quantification of nuclear and cytosolic fluorescence intensities.The best running back by far is Adrian Petterson. No question. This next one is hard.

People say that the Colosseum is in a lousy neighborhood and is too big to sell out every week. According to NFL rules, if a game is not sold out, it cannot be on local TV. If the team were more popular these issues would not be of concern in the first place.They didn't do all the stuff necessary to prepare the