(C) Nterminally HAtagged wildtype (WT) FUS and the indicated point mutants were transiently expressed in HeLa cells; 24 h posttransfection cells were stained with an HAspecific antibody (green), a nuclear counterstain (blue) and analysed by confocal microscopy. Although the WT protein is located almost exclusively in the nucleus, deletion of the Cterminal 13 amino acids ( or replacement of arginine residues by alanine (R521A/R522A/R524A or R514A/R518A) leads to a predominantly cytosolic localization. Scale bar, 20 (D) Quantification of nuclear and cytosolic fluorescence intensities.21:4 For this we tell you by the word of the Lord, that we who are alive, who are left to the coming of the Lord, will in no way precede those who have died. For the Lord himself will come down from heaven with a shout, with the voice of the archangel, and with Gods trumpet. The dead in Christ will rise first, then we who are alive, who are left, will be caught up together with them in the clouds, to mee