Several transduction experiments were performed and stable clonal lines were subsequently isolated from transduced cells. For singlecell sorting, GFP+transduced Hut78 cells were sorted into the wells of a 96well plate by FACS. Subsequent clone formation (presence of 2 refractile cells) and clone size (total number of refractile cells) in wells seeded with single viable propidium iodide negative (PI) cells was assessed by direct visual inspection using an inverted microscope..For the future, the researchers plan to study use of a less expensive nanosecond laser to replace the ultrafast femtosecond instrument used in the research. They also plan to optimize the carbon nanoparticles so that nearly all of them are consumed during the exposure to laser light. Leftover carbon nanoparticles in the body should produce no harmful effects, though the body may be unable to eliminate them, Prausnitz noted..

JaMarcus Russell has experienced an incredible fall from grace since playing q