Top of pageMaterials and methodsRetroviral vector and virus productionThe RPG vector was constructed based on the RVH1 vector as described.21 The cytomegalovirus promoter was replaced with the phosphogluco kinase (PGK) promoter, and the human CD4 gene was replaced with an enhanced green fluorescent protein as a selection marker. The human H1 promoter was maintained upstream of the PGK promoter. The oligonucleotides encoding the AHI1 small interfering RNAs (siRNA) that inhibited AHI1 expression (AHIsh4) were 5'GATCCCCGTGATGATCCCGACACTATTTCAAGAGAATAGTGTCGGGAT CATCACTTTTTA3' and 5'AGCTTAAAAAGTGATGATCCCGACACTATTCTCTTGAAATAGTGTCGG GATCATCACGGG3'.Howard, it's rumoured, was once disrespectful of Nelson Mandela, even if nobody seems to know when or how, and this also must have been a while ago, because Howard was also responsible in November 1999 for making Mandela an honorary companion in the Order of Australia. Perhaps Nyoka is sincere in his objections; perhaps not. He has so far exhibite