IYS [the nickname for Immerse Yourself in Service] is a totally studentrun organization that does service trips during Fall and Spring breaks. Interested students must fill out an application and rank the sites in order of preference, and then the student leaders choose who they would like to come on their trip. Four sophomores, one junior, two seniors, and a GPS named Tina stuffed themselves in a rented minivan to embark on what would soon become the longest car ride of my life."Like I told the guys, eight, nine, 10 were our best overall team practices," Rudolph said. "So the positives are that we got better as the spring went on. Now, it remains to be seen where we were at for practice No.

Several transduction experiments were performed and stable clonal lines were subsequently isolated from transduced cells. For singlecell sorting, GFP+transduced Hut78 cells were sorted into the wells of a 96well plate by FACS. Subsequent clone formation (presence of 2 re