Western blot analysesCells were lysed in phosphorylation solubilization buffer as described previously.28 Protein lysates were then separated on NuPAGE Novex bis gels (Invitrogen) and the filters were probed with a polyclonal antiAhi1 antibody (Cterminal) or a polyclonal Nterminal AHI1 antibody, IMX3395 (IMGENEX, San Diego, CA, USA). Western blot analysis was also performed using an antiTNF antibody (H156, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and a monoclonal human antiactin antibody (Sigma, Saint Louis, MO, USA). Relative expression levels of AHI1 and TNF were normalized to expression levels of human actin after quantification with ImageQuant software..It's a feeling I get sometimes that Test cricket is now the Mother Teresa of cricket. You have to say at the awards ceremony that Mother Teresa is the ultimate role model, even if you just about knew that she came from Albania and went to Kolkata. Is Test cricket becoming a Mother Teresa thing where people want to say what