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Top of pageResultsStable suppression of AHI1 expression decreases autocrine production of cytokines in Hut78 cellsTo investigate directly whether deregulated expression of AHI1 in human leukemic cells contributes to their transformed properties, knockdown of AHI1 expression in Hut78 cells, a cell line that shows highly deregulated AHI1 expression,5 was performed using retroviralmediated RNAi.22, 23 Hut78 cells are characterized by several interesting transforming phenotypes, including growth factor independence, autocrine production of IL2, IL4, TNF and interferon (IFN), and induc