TDP43 subcellular localization and solubility are regulated by oxidative stressTo determine if oxidative stress implicated in neurodegenerative diseases triggers alterations in TDP43 subcellular localization and/or solubility, Cos7 cells were treated with various stressors including hydrogen peroxide (H2O2) and arsenite, which generate ROS through distinct mechanisms (Valko et al, 2005; Calabrese et al, 2010; Jomova et al, 2010). Consistent with the previous reports that TDP43 localizes to SGs, H2O2 induced a rapid translocation of TDP43 into cytosolic SGs, as determined by colocalization with the SG marker TIAR (Kedersha et al, 1999) (Figure 1Aa and B). In contrast, although arsenite treatment led to robust formation of TIARpositive SGs, TDP43 was not prominently detected within these SGs (Figure 1Ad and B), supporting observations that the majority of TDP43 remains nuclear localized in response to arsenite treatment (LiuYesucevitz et al, 2010; Dewey et al, 2011).RETRO PREDICTION: